Melany Jumbo, Quito, Ecuador 🇪🇨

Week 9

PART A

  1. Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell free expression is more beneficial than cell production.

A:

Advantages Cases
Fast responding time: as it doesn’t require the growth and maintenance of live cells. ⏱️ In vitro Protein Production: Allow for the complete biosynthesis of complex natural products. Also they’re robust enough to express large biosynthetic gene clusters. Easy optimization and improved production. Finally, they’re powerful for rapidly detecting and generating new natural products and analogues.
Direct Control over Reaction Conditions: pH, temperature, cofactors, and other parameters can be easily controlled, making it easier to optimize protein production Antibody: It can build MAbs (Monoclonal antibodys) with low cost and reduce the time for the production.

https://www.ime.fraunhofer.de/en/Research_Divisions/business_fields_MB/Plant_biotechnology/RecombinantProt_DSP/Cellfree_protein_synthesis.html

https://pmc-ncbi-nlm-nih-gov.translate.goog/articles/PMC8705439/?_x_tr_sl=en&_x_tr_tl=es&_x_tr_hl=es&_x_tr_pto=sge#:~:text=Las plataformas libres de células tienen muchas ventajas sobre los,en la producción de anticuerpos.

veranosug,+125+Artículo-José+Eleazar+Barboza+Corona.pdf

  1. Describe the main components of a cell-free expression system and explain the role of each component.

A:

Components of a cell-free protein synthesis reaction. The reaction is carried out in a test tube; that is, DNA, amino acids, and energy buffers mix with the molecular machinery present in the cell lysate to initiate transcription and translation for the synthesis of functional proteins.

Components of a cell-free protein synthesis reaction. The reaction is carried out in a test tube; that is, DNA, amino acids, and energy buffers mix with the molecular machinery present in the cell lysate to initiate transcription and translation for the synthesis of functional proteins.

Additional Information:

🔋Energy source: Used to regenerate ATP, such as phosphoenolpyruvate or creatine phosphate.

🧬Cofactors: Such as magnesium, which is essential for protein synthesis.

https://pmc-ncbi-nlm-nih-gov.translate.goog/articles/PMC8705439/?_x_tr_sl=en&_x_tr_tl=es&_x_tr_hl=es&_x_tr_pto=sge#:~:text=Las plataformas libres de células tienen muchas ventajas sobre los,en la producción de anticuerpos.