Melany Jumbo, Quito, Ecuador 🇪🇨

<aside> ⚠️ About your Documentation Make sure to document every step of the in-silico and lab experiments. Make sketches, screenshots, notes, drawings - anything that helps you - and others understand the experiment. Your documentation should help you - and others - to understand the topic. Don’t be afraid to add things that don’t work. Show your failures - and how you did overcome them. Your Documentation should be a description of the amazing journey you are on!

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Part 0: Basics of Gel Electrophoresis

• [x] Watch all lecture and recitation videos.
  • [x] Optionally watch bootcamp

Part 1: Benchling & In-silico Gel Art

See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:

• [x] Make a free account at benchling.com
• [x] Import the Lambda DNA.
• [x] Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI
• [x] Create a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks.

https://benchling.com/s/seq-PLyzguYVSTJXSDegWEea?m=slm-0dPYsC0nvk6T5WbFO422

How I made it:

Result:

For me this shape is something like this: Rows 4 and 5 are the handles of a flag that is seen from row 4 to the left and from row 5 to the right a gardening tool.

Part 3: DNA Design Challenge

<aside> ⚠️ Mandatory for MIT/Harvard Students and Committed Listeners. Due start of recitation Wed Feb 21

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